Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth

被引：39

作者：

Gloux, Karine

Leclerc, Marion

Iliozer, Harout

L'Haridon, Rene

Manichanh, Chaysavanh

Corthier, Gerard

Nalin, Renaud

Blottiere, Herve M.

Dore, Joel

机构：

[1] INRA, Unite Ecol & Physiol Syst Digest, F-78352 Jouy En Josas, France

[2] INRA, Unite Virol & Immunol Mol, F-78352 Jouy En Josas, France

[3] LibraGen, F-31400 Toulouse, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 11期

关键词：

D O I：

10.1128/AEM.02204-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.

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页码：3734 / 3737

页数：4

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