The 30S ribosomal P site: a function of 16S rRNA

被引：23

作者：

Noller, HF ^{[1
]}

Hoang, L ^{[1
]}

Fredrick, K ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA

来源：

FEBS LETTERS | 2005年 / 579卷 / 04期

关键词：

ribosome; 16S rRNA; ribosomal proteins; S9; S13; tRNa;

D O I：

10.1016/j.febslet.2004.11.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 30S ribosomal P site serves several functions in translation. It must specifically bind initiator tRNA during formation of the 30S initiation complex; bind the anticodon stem-loop of peptidyl-tRNA during the elongation phase; and help to maintain the translational reading frame when the A site is unoccupied. Early experiments provided evidence that 16S rRNA was an important component of the 30S P site. Footprinting and crosslinking studies later implicated specific nucleotides in interactions with tRNA. The crystal structures of the 30S subunit and 70S ribosome-tRNA complexes confirmed the interactions between 16S rRNA and tRNA, but also revealed contacts between tRNA and the C-terminal tails of proteins S9 and S13. Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：855 / 858

页数：4

共 31 条

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