Metal determination and quantification in biological material using particle-induced X-ray emission

被引:26
作者
Bertrand, M
Weber, G
Schoefs, B
机构
[1] Conservatoire Natl Arts & Metiers, Natl Inst Marine Sci & Tech, F-50103 Cherbourg, France
[2] Univ Liege, Inst Phys Nucl Atom & Spect, B-4000 Liege, Belgium
[3] Univ Grenoble 1, CNRS, F-38041 Grenoble, France
关键词
AAS; atomic absorption spectroscopy; AES; atomic emission spectroscopy; CE; capillary electrophoresis; GF-AAS; graphite furnace atomic absorption; spectroscopy; HPLC; high-performance liquid chromatography; ICP-MS; inductively coupled plasma-mass spectrometry; IEF; isoelectrofocusing; PAGE; polyacrylamide gel electrophoresis; PIGE; particle-induced gamma-ray emission; PIXE; particleinduced X-ray emission; SDS; sodium dodecyl sulphate; XRF; X-ray fluorescence;
D O I
10.1016/S0165-9936(03)00405-9
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Metal monitoring is one of the major concern of biological and biomedicine research. The particle-Induced X-ray emission (PIXE) method allows a sensitive, multi-elementary and non-destructive analysis of crude samples or purified molecules. A brief description of the method is presented and is followed by a short review of biological applications. The last part emphasises the interest in PIXE for metalloprotein analysis after denaturing and non-denaturing electrophoresis. (C) 2003 Published by Elsevier Science B.V.
引用
收藏
页码:254 / 262
页数:9
相关论文
共 47 条
[41]  
Theophanides T., 1987, SPECTROSCOPY BIOL MO, P433
[42]   Zn(II) dependence of the Aeromonas hydrophila AE036 metallo-beta-lactamase activity and stability [J].
Valladares, MH ;
Felici, A ;
Weber, G ;
Adolph, HW ;
Zeppezauer, M ;
Rossolini, GM ;
Amicosante, G ;
Frere, JM ;
Galleni, M .
BIOCHEMISTRY, 1997, 36 (38) :11534-11541
[43]   Fluid inclusions in quartz from oceanic hydrothermal specimens and the Bingham, Utah porphyry-Cu deposit: a study with PIXE and SXRF [J].
Vanko, DA ;
Bonnin-Mosbah, M ;
Philippot, P ;
Roedder, E ;
Sutton, SR .
CHEMICAL GEOLOGY, 2001, 173 (1-3) :227-238
[44]  
Vogt C, 1999, J FORENSIC SCI, V44, P819
[45]   On-line coupling of PIXE with capillary zone electrophoresis (CZE) [J].
Vogt, J ;
Vogt, C .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1996, 108 (1-2) :133-135
[46]  
WEBER G, 1996, INT J PIXE, V6, P215
[47]   Is d-Al-Cu-Co a random tiling? [J].
Zeger, G ;
Plachke, D ;
Carstanjen, HD ;
Trebin, HR .
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING, 2000, 294 :291-294