Tetrahymena thermophila: Analysis of phospholipids and phosphonolipids by high-field H-1-NMR

被引：19

作者：

Adosraku, RK

Smith, JD

Nicolaou, A

Gibbons, WA

机构：

[1] UNIV LONDON,SCH PHARM,IND CTR PHARMACEUT RES,LONDON WC1N 1AX,ENGLAND

[2] UNIV MASSACHUSETTS,DEPT CHEM,N DARTMOUTH,MA 02747

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1996年 / 1299卷 / 02期

关键词：

lipid analysis; NMR; phospholipid; phosphonolipid; (T-thermophila);

D O I：

10.1016/0005-2760(95)00181-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The phospholipids of control and lipid-modified Tetrahymena thermophila were identified and quantified, using 1-D and 2-D COSY proton NMR spectroscopy on intact lipids, before and after HPLC separation. The results are comparable to those obtained using classical lipid analytical techniques. The results indicate that the study of enzyme pathways and other metabolic processes involving phospholipids in Tetrahymena and related protozoa can be carried out using proton NMR spectroscopy as the investigating technique.

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页码：167 / 174

页数：8

相关论文

共 21 条

[1] PROTON NMR LIPID PROFILE OF LEISHMANIA-DONOVANI PROMASTIGOTES [J].

ADOSRAKU, RK ;

ANDERSON, MM ;

ANDERSON, GJ ;

CHOI, G ;

CROFT, SL ;

YARDLEY, V ;

PHILLIPSON, JD ;

GIBBONS, WA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 62 (02) :251-262

[2] ALTERATION OF LIPID-COMPOSITION IN 2 TAXA OF TETRAHYMENA BY 1ST AND REPEATED INSULIN-TREATMENT [J].

BENCSATH, M ;

PICK, J ;

CSABA, G .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1991, 98 (2-3) :233-237

[3]

BENCSATH M, 1988, SEP P INT C BIOCH, P109

[4] NMR LIPID PROFILES OF CELLS, TISSUES AND BODY-FLUIDS .1. 1D AND 2D PROTON NMR OF LIPIDS FROM RAT-LIVER [J].

CASU, M ;

ANDERSON, GJ ;

CHOI, G ;

GIBBONS, WA .

MAGNETIC RESONANCE IN CHEMISTRY, 1991, 29 (06) :594-602

[5]

Hilderbrand R.L., 1983, P5

[6] INVOLVEMENT OF THE PHOSPHOINOSITOL (PI) SYSTEM IN THE MECHANISM OF HORMONAL IMPRINTING [J].

KOVACS, P ;

CSABA, G .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 170 (01) :119-126

[7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF ADULT HUMAN BRONCHOALVEOLAR LAVAGE - ASSAY FOR PHOSPHOLIPID LUNG PROFILE [J].

PISON, U ;

GONO, E ;

JOKA, T ;

OBERTACKE, U ;

OBLADEN, M .

JOURNAL OF CHROMATOGRAPHY, 1986, 377 :79-89

[8] SEPARATION OF PHOSPHOLIPIDS AND PHOSPHONOLIPIDS OF TETRAHYMENA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

SMITH, JD ;

MELLO, CM ;

OREILLY, DJ .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 431 (02) :395-399

[9] EFFECT OF A PHOSPHONIC ACID ANALOG OF CHOLINE PHOSPHATE ON PHOSPHOLIPID-METABOLISM IN TETRAHYMENA [J].

SMITH, JD ;

GIEGEL, DA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1982, 213 (02) :595-601

[10] EFFECT OF MODIFICATION OF MEMBRANE PHOSPHOLIPID-COMPOSITION ON THE ACTIVITY OF PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE OF TETRAHYMENA [J].

SMITH, JD .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 223 (01) :193-201