Near-field fluorescence microscopy of cells

被引:42
作者
Enderle, T [1 ]
Ha, T [1 ]
Chemla, DS [1 ]
Weiss, S [1 ]
机构
[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Mat Sci, Mol Design Inst, Berkeley, CA 94720 USA
关键词
near-held optical microscopy; microscopic methods; specifically for biological specimens; spectroscopy in NFOM; green fluorescent protein; red-blood cells; malaria;
D O I
10.1016/S0304-3991(97)00075-2
中图分类号
TH742 [显微镜];
学科分类号
摘要
We demonstrate the surface sensitivity of near-field scanning optical microscopy by fluorescence imaging of membrane and bulk proteins in cells. We discuss instrument design considerations for successful cell-biology work with NSOM and show that the technique is most suited for studying membrane proteins. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:303 / 309
页数:7
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