A putative signal recognition particle receptor a subunit (SR alpha) homologue is expressed in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius

被引：6

作者：

Moll, R ^{[1
]}

Schmidtke, S ^{[1
]}

Schaefer, G ^{[1
]}

机构：

[1] UNIV LUBECK, INST BIOCHEM, D-23538 LUBECK, GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 137卷 / 01期

关键词：

Sulfolobus acidocaldarius; archaeon; protein translocation; signal recognition particle receptor alpha subunit; transcription; SR alpha fusion protein;

D O I：

10.1111/j.1574-6968.1996.tb08081.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 1.64 kb genomic DNA sequence from the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius is composed of two adjacent genes, The first functionally unassigned open reading frame (orf-l) comprises 450 base pairs. The second 1.1 kb large open reading frame encodes the putative signal recognition particle receptor oc subunit (SR alpha). Both genes are expressed under the heterotrophic growth conditions of the organism. The main transcript of orf-l appears as a monocistronic RNA in Northern hybridization. With regard to SR alpha the transcription pattern was investigated by reverse transcription polymerase chain reaction and primer extension analysis. A polyclonal antiserum directed against E, coli lacZ'/Sulfolobus SR alpha fusion protein detects a 40.5 kDa protein (p41) in agreement with the 41.4 kDa as deduced from the nucleotide sequence.

引用

页码：51 / 56

页数：6

共 15 条

[1]

AIBA H, 1981, J BIOL CHEM, V256, P1905

[2] DNA SEQUENCING WITH DIRECT BLOTTING ELECTROPHORESIS [J].