Distribution and concentration of urocortin, and effect of adrenalectomy on its content in rat hypothalamus

被引：72

作者：

Oki, Y ^{[1
]}

Iwabuchi, M ^{[1
]}

Masuzawa, M ^{[1
]}

Watanabe, F ^{[1
]}

Ozawa, M ^{[1
]}

Iino, K ^{[1
]}

Tominaga, T ^{[1
]}

Yoshimi, T ^{[1
]}

机构：

[1] Hamamatsu Univ Sch Med, Dept Med, Div 2, Hamamatsu, Shizuoka 43131, Japan

来源：

LIFE SCIENCES | 1998年 / 62卷 / 09期

关键词：

urocortin; corticotropin-releasing factor; adrenocorticotropin; adrenalectomy; hypothalamus; pituitary;

D O I：

10.1016/S0024-3205(97)01182-X

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We developed a specific and sensitive radioimmunoassay (RIA) for rat urocortin (rUcn) and investigated the tissue distribution and concentration of immunoreactive (IR-)Ucn in rats. Antiserum was obtained by immunizing rabbits with synthetic rUcn21-35 coupled with bovine thyroglobulin. (125)I-[Tyr]18-rUcn19-37 was used as the tracer. The RIA detected synthetic rUcn1-40 as low as 0.4 fmol/tube, and did not cross-react with other corticotropin-releasing factor-related peptides. IR-Ucn was widely distributed in central nervous system, endocrine organs, and digestive system. Its concentration was highest in pituitary (11.0 +/- 1.36 pmol/g.w.w., mean +/- SEM, n=4). Reverse-phase HPLC revealed that hypothalamic IR-Ucn had similar chromatographic mobility to synthetic rUcn1-40. However, bilateral adrenalectomy did not influence the hypothalamic IR-Ucn content. Our results suggest that Ucn may play important roles in various tissues in normal rats, but not behave as a hypothalamic hypophysiotropic factor in mediating adrenocorticotropin secretion in adrenalectomized rats.

引用

页码：807 / 812

页数：6

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