Piezoelectric immunosensor based on magnetic nanoparticles with simple immobilization procedures

被引：61

作者：

Li, JS ^{[1
]}

He, XX ^{[1
]}

Wu, ZY ^{[1
]}

Wang, KM ^{[1
]}

Shen, GL ^{[1
]}

Yu, RQ ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2003年 / 481卷 / 02期

基金：

中国国家自然科学基金;

关键词：

magnetic bio-nanoparticle; piezoelectric immunosensor; protein immobilization;

D O I：

10.1016/S0003-2670(03)00089-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method for immobilizing antibodies (antigens) based on magnetic nanoparticles has been proposed for piezoelectric immunoassay. The goat-anti-IgG antibody (IgGAb) as the model analyte was first covalently immobilized to magnetic nanoparticles, which were surface modified with amino-groups. The magnetic bio-nanoparticles (MBN-s) formed were attached to the surfaces of quartz crystal with the help of a permanent magnet. The detection of immunoglobulin G (IgG) was performed with the sensor prepared. The process of immobilization and immunoreaction was monitored by frequency recording. From the SEM images of the sensor surface before and after immobilization of MBN, one can see that the MBN was homogeneously adsorbed on sensor surface. The piezoelectric immunosensor can determine IgG in the range of 0.6-34.9 mug ml(-1) with a detection limit of 0.36 mug ml(-1). The MBN and immunocomplex layer can easily be removed simply by taking away the magnetic field, making the piezoelectric sensor easy to be regenerated. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：191 / 198

页数：8

共 16 条

[1]

BORCH RF, 1971, J AM CHEM SOC, V93, P28970

[2] Assembly of alternating polyelectrolyte and protein multilayer films for immunosensing .2. [J].