On-line monitoring of lipolytic activity by sequential injection analysis

被引：7

作者：

Cos, O ^{[1
]}

Montesinos, JL ^{[1
]}

Lafuente, J ^{[1
]}

Solà, C ^{[1
]}

Valero, F ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Dept Engn Quim ETSE, E-08193 Barcelona, Spain

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 22期

关键词：

Candida rugosa; fermentation; lipolytic activity; on-line monitoring; sequential injection analysis;

D O I：

10.1023/A:1005654304541

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An automated sequential injection analysis using stop-flow technique for the on-line determination of lipolytic activity has been developed. It is based on a colorimetric method using a chromogenic substrate, 1,2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin)-ester. The system permits a linear range analysis between 5-100 lipolytic activity units ml(-1), without external dilution of the sample, a sampling frequency of 5 samples per hour and a relative standard deviation (RSD) of 5%. The analyser has been used for the on-line monitoring of Candida rugosa fed-batch fermentation with excellent performance, regarding its reliability and reproducibility.

引用

页码：1783 / 1788

页数：6

共 17 条

[1]

BAXTER PJ, 1996, ACCOUNTS CHEM RES, V29, P315

[2]

CHEMNITIUS GC, 1990, P 5 EUR C BIOT, P544

[3]

ERDMANN H, 1990, GBF MONOGRAPHS, V16, P425

[4]

GORDILLO MA, 1995, APPL MICROBIOL BIOT, V43, P38, DOI 10.1007/s002530050367

[5]

Gordillo MA, 1998, BIOTECHNOL BIOENG, V60, P156, DOI 10.1002/(SICI)1097-0290(19981020)60:2<156::AID-BIT3>3.0.CO

[6]

2-M

[7] MULTISTEP DETERMINATION OF ENZYME-ACTIVITY BY FLOW-INJECTION AND SEQUENTIAL INJECTION-ANALYSIS - ASSAY OF AMYLOGLUCOSIDASE [J].

HANSEN, EH ;

WILLUMSEN, B ;

WINTHER, SK ;

DRABOL, H .

TALANTA, 1994, 41 (11) :1881-1893

[8] Biotechnological application of Pseudomonas aeruginosa lipase: Efficient kinetic resolution of amines and alcohols [J].

Jaeger, KE ;

Liebeton, K ;

Zonta, A ;

Schimossek, K ;

Reetz, MT .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1996, 46 (02) :99-105

[9] BACTERIAL LIPASES [J].

JAEGER, KE ;

RANSAC, S ;

DIJKSTRA, BW ;

COLSON, C ;

VANHEUVEL, M ;

MISSET, O .

FEMS MICROBIOLOGY REVIEWS, 1994, 15 (01) :29-63

[10] DETECTION AND DETERMINATION OF LIPASE (ACYLGLYCEROL HYDROLASE) ACTIVITY FROM VARIOUS SOURCES [J].

JENSEN, RG .

LIPIDS, 1983, 18 (09) :650-657

← 1 2 →