MULTISTEP DETERMINATION OF ENZYME-ACTIVITY BY FLOW-INJECTION AND SEQUENTIAL INJECTION-ANALYSIS - ASSAY OF AMYLOGLUCOSIDASE

被引：21

作者：

HANSEN, EH

WILLUMSEN, B

WINTHER, SK

DRABOL, H

机构：

[1] Chemistry Department A, The Technical University of Denmark, DK-2800 Lyngby

来源：

TALANTA | 1994年 / 41卷 / 11期

关键词：

D O I：

10.1016/0039-9140(94)00136-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A multi-point assay for determination of the activity of amylglucosidase (AMG) by FIA and SIA is described. The assay is based on two consecutive reactions that are mutually incompatible. both the FIA and SIA procedures allow these two reactions to be completely separated, whereby each of the processes can be individually optimized with respect to operational parameters. Special emphasis is placed on comparing the performance and applicability of the two procedures for this type of assay.

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页码：1881 / 1893

页数：13

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