Combined thrombophilic polymorphisms in women with idiopathic recurrent miscarriage

Objective: To identify associations or interrelations between carriage of the methylenetetrahydrofolate reductase (MTHFR) C677T, the MTHFR A1298C, the factor V Leiden G1691A, the factor 11 prothrombin G20210A, the human platelet antigen (HPA) I C12548T, and the apolipoprotein (APO) B R3500Q polymorphisms and idiopathic recurrent miscarriage (IRM). Design: Prospective case control study. Setting: Academic research institution. Patient(s): One hundred forty-five women with a history of three or more consecutive pregnancy losses before 20 weeks gestation and 101 healthy postmenopausal women with at least two live births and no history of pregnancy loss. Intervention(s): Peripheral venous punctures. Main Outcome Measure(s): Multiplex polymerase chain reaction was performed to identify the different alleles of six candidate genetic risk factors for IRM (MTHFR C677T, MTHFR A1298C, factor V Leiden G1691A, factor 11 prothrombin G20210A, HPA I C12548T, and the APO B R3500Q). Result(s): Allele and genotype frequencies of all polymorphisms were not significantly different between the study and the control groups. Also, no significant associations occurred between combinations of polymorphisms; and the occurrence of IRM. Conclusion(s): Our data fall short of showing any significant association between single polymorphisms of the MTHFR, the Factor V Leiden, the Factor 11 Prothrombin, the HPA I and APO B genes or combinations of these polymorphisms and the occurrence of IRM. (C) 2003 by American Society for Reproductive Medicine.