Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola

被引：111

作者：

Solaiman, Daniel K. Y. ^{[1
]}

Ashby, Richard D. ^{[1
]}

Zerkowski, Jonathan A. ^{[1
]}

Foglia, Thomas A. ^{[1
]}

机构：

[1] Eastern Reg Res Ctr, Agr Res Serv, USDA, Fats Oils & Anim Coprod Res Unit, Wyndmoor, PA 19038 USA

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 09期

关键词：

biosurfactant; Candida bombicola; nitrogen source; soy solubles;

D O I：

10.1007/s10529-007-9407-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A simplified medium containing only soy molasses and oleic acid as ingredients was developed for the production of sophorolipids (SLs) from Candida bombicola. We achieved a product yield of 53 +/- 3 g of purified sophorolipids per liter of starting culture volume, which is 71 +/- 4% of the yield obtained with growth medium that also additionally contains the costly yeast extract and urea as nitrogen source. The large majority of the SL components existed in the lactone form (87%), and the predominant component is SL containing (omega-1)-hydroxyoleic acid as the lipid moiety. The study demonstrated for the first time the usefulness of the low-value soy molasses as a combined nitrogen- and carbon-source for SL production at a reduced cost.

引用

页码：1341 / 1347

页数：7

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