Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells

被引：83

作者：

Hu, YC ^{[1
]}

Hsu, JTA

Huang, JH

Ho, MS

Ho, YC

机构：

[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 300, Taiwan

[2] Acad Sinica, Natl Hlth Res Inst, Div Biotechnol & Pharmaceut Res, Taipei 11529, Taiwan

[3] Acad Sinica, Inst Biomed Sci, Taipei 11529, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 12期

关键词：

baculovirus; enterovirus; 71; insect cell; virus aggregation; virus-like particle;

D O I：

10.1023/A:1024071514438

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The assembly of enterovirus requires the cleavage of P1 polyprotein by protease 3CD into individual structural proteins. Two recombinant baculoviruses were constructed to encode P1 and 3CD of enterovirus 71 (EV71), respectively. The expressed 3CD successfully cleaved P1 in vitro and in vivo. Also, the co-infection in insect cells resulted in crystalline virus-like particle structures morphologically resembling the authentic EV71 aggregates, which are reported for the first time.

引用

页码：919 / 925

页数：7

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