Gene silencing of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis by RNA interference

被引：36

作者：

Miyoshi, T

Tsuji, N

Islam, MK

Kamio, T

Fujisaki, K

机构：

[1] Natl Agr Res Org, Natl Inst Anim Hlth, Parasit Dis Lab, Tsukuba, Ibaraki 3050856, Japan

[2] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2004年 / 66卷 / 11期

关键词：

Haemaphysalis longicornis; RNA interference; serine proteinase;

D O I：

10.1292/jvms.66.1471

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

RNA interference (RNAi) has been recently exploited to determine gene function by degrading specific mRNAs in several eukaryotic organisms. We constructed a double stranded RNA (dsRNA) from a previously cloned Haemaphysalis longicornis serine proteinase (HISP) gene to test the importance of the function of the HISP gene product during blood-feeding. Growth of unfed ticks treated with HISP dsRNA was significantly inhibited compared to that of PBS-treated ticks. This inhibition was supported by the level of HISP mRNA. HISP may play a crucial role for blood-feeding in these ticks. This is the first report on gene silencing of a functional serine proteinase in hard ticks.

引用

页码：1471 / 1473

页数：3

共 20 条

[1] RNA interference: applicability in tick research [J].