Mutation of L4 ribosomal protein conferring unusual macrolide resistance in two independent clinical isolates of Staphylococcus aureus

被引：21

作者：

Prunier, AL

Trong, HN

Tande, D

Segond, C

Leclercq, R

机构：

[1] Univ Caen, Equipe EA 2128, Caen, France

[2] CHU Morvan, Microbiol Serv, Brest, France

[3] CHU, Microbiol Serv, Toulouse, France

来源：

MICROBIAL DRUG RESISTANCE | 2005年 / 11卷 / 01期

关键词：

D O I：

10.1089/mdr.2005.11.18

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of this study was to characterize the resistance mechanisms of two strains of Staphylococcus aureus UCN42 and UCN43 isolated from cystic fibrosis patients that displayed an unusual phenotype of resistance to macrolides. The strains were resistant to erythromycin and the 16-membered macrolide spiramycin but susceptible to clindamycin. None of the strains contained erm or msr(A) genes by PCR. Sequencing of genes encoding ribosomal targets of macrolides revealed mutations in conserved regions of the L4 ribosomal protein, a R168S mutation for S. aureus UCN43 and mutations at positions G69A and T70P for both strains. This observation extends previous reports of similar mutations in streptococci and mycoplasma to S. aureus.

引用

收藏

页码：18 / 20

页数：3

相关论文

共 12 条

[1] Macrolide resistance phenotypes and genotypes in French clinical isolates of Streptococcus pneumoniae [J].

Angot, P ;

Vergnaud, M ;

Auzou, M ;

Leclercq, R .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2000, 19 (10) :755-758

[2] In vitro activities of telithromycin, linezolid, and quinupristin-dalfopristin against Streptococcus pneumoniae with macrolide resistance due to ribosomal mutations [J].

Farrell, DJ ;

Morrissey, I ;

Bakker, S ;

Buckridge, S ;

Felmingham, D .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (08) :3169-3171

[3] The polypeptide tunnel system in the ribosome and its gating in erythromycin resistance mutants of L4 and L22 [J].

Gabashvili, IS ;

Gregory, ST ;

Valle, M ;

Grassucci, R ;

Worbs, M ;

Wahl, MC ;

Dahlberg, AE ;

Frank, J .

MOLECULAR CELL, 2001, 8 (01) :181-188

[4] Mechanisms of resistance to macrolides and lincosamides: Nature of the resistance elements and their clinical implications [J].

Leclercq, R .

CLINICAL INFECTIOUS DISEASES, 2002, 34 (04) :482-492

[5] Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci [J].

Lina, G ;

Quaglia, A ;

Reverdy, ME ;

Leclercq, R ;

Vandenesch, F ;

Etienne, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (05) :1062-1066

[6] Resistance to macrolides in clinical isolates of Streptococcus pyogenes due to ribosomal mutations [J].

Malbruny, B ;

Nagai, K ;

Coquemont, M ;

Bozdogan, B ;

Tambic Andrasevic, A ;

Hupkova, H ;

Leclercq, R ;

Appelbaum, PC .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 49 (06) :935-939

[7] In vitro selection and characterization of resistance to macrolides and related antibiotics in Mycoplasma pneumoniae [J].

Pereyre, S ;

Guyot, C ;

Renaudin, H ;

Charron, A ;

Bébéar, C ;

Bébéar, CM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (02) :460-465

[8] High rate of macrolide resistance in Staphylococcus aureus strains from patients with cystic fibrosis reveals high proportions of hypermutable strains [J].

Prunier, AL ;

Malbruny, B ;

Laurans, M ;

Brouard, J ;

Duhamel, JF ;

Leclercq, R .

JOURNAL OF INFECTIOUS DISEASES, 2003, 187 (11) :1709-1716

[9] Clinical isolates of Staphylococcus aureus with ribosomal mutations conferring resistance to macrolides [J].

Prunier, AL ;

Malbruny, B ;

Tandé, D ;

Picard, B ;

Leclercq, R .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (09) :3054-3056

[10] INDUCIBLE ERYTHROMYCIN RESISTANCE IN STAPHYLOCOCCI IS ENCODED BY A MEMBER OF THE ATP-BINDING TRANSPORT SUPERGENE FAMILY [J].

ROSS, JI ;

EADY, EA ;

COVE, JH ;

CUNLIFFE, WJ ;

BAUMBERG, S ;

WOOTTON, JC .

MOLECULAR MICROBIOLOGY, 1990, 4 (07) :1207-1214