Sequential fractionation procedure for the identification of potentially cytochrome P4501A-inducing compounds

被引：39

作者：

Brack, W

Kind, T

Hollert, H

Schrader, S

Möder, M

机构：

[1] UFZ Helmholtz Ctr Environm Res, Environm Res Ctr, Dept Chem Ecotoxicol, D-04318 Leipzig, Germany

[2] Heidelberg Univ, Dept Zool 1, D-69120 Heidelberg, Germany

[3] UFZ Helmholtz Ctr Environm Res, Environm Res Ctr, Dept Analyt Chem, D-04318 Leipzig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 986卷 / 01期

关键词：

multistep fractionation; preparative chromatography; cytochrome P4501A-inducing compounds;

D O I：

10.1016/S0021-9673(02)01909-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A multistep fractionation procedure for the separation of nonpolar aromatic compounds with respect to cytochrome P4501A induction is presented. Normal-phase HPLC on nitrophenylpropyl silica and cyanopropyl silica was tested for group-specific separation as a first fractionation step. Subsequent individual compound-specific PAH fractionation was done by means of reversed-phase HPLC. Electron-donor-acceptor HPLC and size-exclusion chromatography were applied to separate PAHs, PCBs, PCNs and PCDD/Fs according to their number of aromatic carbon atoms, their hydrophobicity, their degree of chlorination, their planarity and their molecular size. The method was validated for complex environmental mixtures on the basis of two sediment extracts. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：55 / 66

页数：12

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