Labelling of mammalian cells for visualisation by MRI

被引：25

作者：

Bernsen, Monique R. ^{[1
,2
]}

Moelker, Amber D. ^{[2
]}

Wielopolski, Piotr A. ^{[2
]}

van Tiel, Sandra T. ^{[2
]}

Krestin, Gabriel P. ^{[2
]}

机构：

[1] Erasmus MC, Dept Radiol, NL-3000 CA Rotterdam, Netherlands

[2] Erasmus MC Univ Med Ctr Rotterdam, Dept Radiol, NL-3015 GE Rotterdam, Netherlands

来源：

EUROPEAN RADIOLOGY | 2010年 / 20卷 / 02期

关键词：

Cell labelling; Contrast agents; Cell tracking; Apoptosis; MRI; MESENCHYMAL STEM-CELLS; IRON-OXIDE PARTICLES; MAGNETIC-RESONANCE TRACKING; IN-VIVO TRACKING; HEMATOPOIETIC PROGENITOR CELLS; CONTRAST AGENTS; BONE-MARROW; TAT PEPTIDE; T-CELLS; TRANSFECTION AGENT;

D O I：

10.1007/s00330-009-1540-1

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Through labelling of cells with magnetic contrast agents it is possible to follow the fate of transplanted cells in vivo with magnetic resonance imaging (MRI) as has been demonstrated in animal studies as well as in a clinical setting. A large variety of labelling strategies are available that allow for prolonged and sensitive detection of the labelled cells with MRI. The various protocols each harbour specific advantages and disadvantages. In choosing a particular labelling strategy it is also important to ascertain that the labelling procedure does not negatively influence cell functionality, for which a large variety of assays are available. In order to overcome the challenges still faced in fully exploiting the benefits of in vivo cell tracking by MRI a good understanding and standardisation of the procedures and assays used will be crucial.

引用

页码：255 / 274

页数：20

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