Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging

被引：108

作者：

Philip, J ^{[1
]}

Carlsson, K

机构：

[1] Royal Inst Technol, Dept Math, SE-10044 Stockholm, Sweden

[2] Royal Inst Technol, Stockholm Ctr Phys Astron & Biotechnol, SE-10044 Stockholm, Sweden

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 2003年 / 20卷 / 02期

关键词：

D O I：

10.1364/JOSAA.20.000368

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detected photons, we find that frequency-domain and time-domain methods are equally good. The correct choice of detection technique and its parameters is important for obtaining good results. (C) 2003 Optical Society of America.

引用

页码：368 / 379

页数：12

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