Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging

被引：108

作者：

Philip, J ^{[1
]}

Carlsson, K

机构：

[1] Royal Inst Technol, Dept Math, SE-10044 Stockholm, Sweden

[2] Royal Inst Technol, Stockholm Ctr Phys Astron & Biotechnol, SE-10044 Stockholm, Sweden

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 2003年 / 20卷 / 02期

关键词：

D O I：

10.1364/JOSAA.20.000368

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We deduce the signal-to-noise ratio for fluorescence lifetime imaging when using frequency-domain methods. We assume mono-exponential decay and quantum-noise-limited performance. The results are compared with Monte Carlo simulations with good agreement. We also compare our results with previous investigations of time-domain methods for fluorescence lifetime imaging. For a given number of detected photons, we find that frequency-domain and time-domain methods are equally good. The correct choice of detection technique and its parameters is important for obtaining good results. (C) 2003 Optical Society of America.

引用

页码：368 / 379

页数：12

共 30 条

[21] FLUORESCENCE LIFETIME IMAGING MICROSCOPY (FLIMSCOPY) - METHODOLOGY DEVELOPMENT AND APPLICATION TO STUDIES OF ENDOSOME FUSION IN SINGLE CELLS [J].

OIDA, T ;

SAKO, Y ;

KUSUMI, A .

BIOPHYSICAL JOURNAL, 1993, 64 (03) :676-685

[22]

PISTON DW, 1992, TIME RESOLVED LASER, V1640, P379

[23]

Sauer M, 1998, BIOIMAGING, V6, P14, DOI 10.1002/1361-6374(199803)6:1<14::AID-BIO3>3.0.CO

[24]

2-O

[25]

So P. T. C., 1995, Bioimaging, V3, P49, DOI 10.1002/1361-6374(199506)3:2<49::AID-BIO1>3.3.CO

[26]

2-5

[27] MEASUREMENTS OF SUBNANOSECOND FLUORESCENCE LIFETIMES WITH A CROSS-CORRELATION PHASE FLUOROMETER [J].

SPENCER, RD ;

WEBER, G .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1969, 158 (A1) :361-&

[28] Three dimensional image restoration in fluorescence lifetime imaging microscopy [J].

Squire, A ;

Bastiaens, PIH .

JOURNAL OF MICROSCOPY, 1999, 193 :36-49

[29]

Sytsma J, 1998, J MICROSC-OXFORD, V191, P39

[30] MICROVOLUME TIME-RESOLVED FLUORESCENCE SPECTROSCOPY USING A CONFOCAL SYNCHROTRON-RADIATION MICROSCOPE [J].

VANDEROORD, CJR ;

GERRITSEN, HC ;

ROMMERTS, FFG ;

SHAW, DA ;

MUNRO, IH ;

LEVINE, YK .

APPLIED SPECTROSCOPY, 1995, 49 (10) :1469-1473

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