A yeast mitochondrial membrane methyltransferase-like protein can compensate for oxa1 mutations

被引:34
作者
Lemaire, C [1 ]
Guibet-Grandmougin, F [1 ]
Angles, D [1 ]
Dujardin, G [1 ]
Bonnefoy, N [1 ]
机构
[1] CNRS, Ctr Genet Mol, UPR 2167, F-91198 Gif Sur Yvette, France
关键词
D O I
10.1074/jbc.M404861200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Members of the Oxa1p/Alb3/YidC family mediate the insertion of various organelle or bacterial hydrophobic proteins into membranes. They present at least five transmembrane segments (TM) linked by hydrophilic domains located on both sides of the membrane. To examine how Oxa1p structure relates to its function, we have introduced point mutations and large deletions into various domains of the yeast mitochondrial protein. These mutants allowed us to show the importance of the first TM domain as well as a synergistic interaction between the first loop and the C-terminal tail, which both protrude into the matrix. These mutants also led to the isolation of a high copy suppressor, OMS1, which encodes a member of the methyltransferase family. Overexpression of OMS1 seems to increase the steady-state level of both the mutant and wild-type Oxa1p. We show that Oms1p is a mitochondrial inner membrane protein inserted independently of Oxa1p. Oms1p presents one TM and a N-in C-out topology with the C-terminal domain carrying the methyltransferase-like domain. A conserved motif within this domain is essential for the suppression of oxa1 mutations. We discuss the possible role of Oms1p on Oxa1p intermembrane space domain.
引用
收藏
页码:47464 / 47472
页数:9
相关论文
共 53 条
[1]   The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase [J].
Altamura, N ;
Capitanio, N ;
Bonnefoy, N ;
Papa, S ;
Dujardin, G .
FEBS LETTERS, 1996, 382 (1-2) :111-115
[2]   PET1402, A NUCLEAR GENE REQUIRED FOR PROTEOLYTIC PROCESSING OF CYTOCHROME-OXIDASE SUBUNIT-2 IN YEAST [J].
BAUER, M ;
BEHRENS, M ;
ESSER, K ;
MICHAELIS, G ;
PRATJE, E .
MOLECULAR & GENERAL GENETICS, 1994, 245 (03) :272-278
[3]   CLONING OF A HUMAN GENE INVOLVED IN CYTOCHROME-OXIDASE ASSEMBLY BY FUNCTIONAL COMPLEMENTATION OF AN OXA1(-) MUTATION IN SACCHAROMYCES-CEREVISIAE [J].
BONNEFOY, N ;
KERMORGANT, M ;
GROUDINSKY, O ;
MINET, M ;
SLONIMSKI, PP ;
DUJARDIN, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (25) :11978-11982
[4]   OXA1, A SACCHAROMYCES-CEREVISIAE NUCLEAR GENE WHOSE SEQUENCE IS CONSERVED FROM PROKARYOTES TO EUKARYOTES CONTROLS CYTOCHROME-OXIDASE BIOGENESIS [J].
BONNEFOY, N ;
CHALVET, F ;
HAMEL, P ;
SLONIMSKI, PP ;
DUJARDIN, G .
JOURNAL OF MOLECULAR BIOLOGY, 1994, 239 (02) :201-212
[5]   Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is controlled by the nucleotide sequence specifying the pre-Cox2p leader peptide [J].
Bonnefoy, N ;
Bsat, N ;
Fox, TD .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (07) :2359-2372
[6]   ABC1, A NOVEL YEAST NUCLEAR GENE HAS A DUAL FUNCTION IN MITOCHONDRIA - IT SUPPRESSES A CYTOCHROME-B MESSENGER-RNA TRANSLATION DEFECT AND IS ESSENTIAL FOR THE ELECTRON-TRANSFER IN THE BC1 COMPLEX [J].
BOUSQUET, I ;
DUJARDIN, G ;
SLONIMSKI, PP .
EMBO JOURNAL, 1991, 10 (08) :2023-2031
[7]   STRUCTURE-FUNCTION-RELATIONSHIPS OF THE MITOCHONDRIAL BC(1) COMPLEX IN TEMPERATURE-SENSITIVE MUTANTS OF THE CYTOCHROME-B GENE, IMPAIRED IN THE CATALYTIC CENTER-N [J].
BRASSEUR, G ;
COPPEE, JY ;
COLSON, AM ;
BRIVETCHEVILLOTTE, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (49) :29356-29364
[8]   The Oxa2 protein of Neurospora crassa plays a critical role in the biogenesis of cytochrome oxidase and defines a ubiquitous subbranch of the Oxa1/YidC/Alb3 protein family [J].
Funes, S ;
Nargang, FE ;
Neupert, W ;
Herrmann, J .
MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (04) :1853-1861
[9]   Saccharomyces cerevisiae mitochondria lack a bacterial-type Sec machinery [J].
Glick, BS ;
VonHeijne, G .
PROTEIN SCIENCE, 1996, 5 (12) :2651-2652
[10]   THE NAM1/MTF2 NUCLEAR GENE-PRODUCT IS SELECTIVELY REQUIRED FOR THE STABILITY AND/OR PROCESSING OF MITOCHONDRIAL TRANSCRIPTS OF THE ATP6 AND OF THE MOSAIC, COX1 AND CYTB GENES IN SACCHAROMYCES-CEREVISIAE [J].
GROUDINSKY, O ;
BOUSQUET, I ;
WALLIS, MG ;
SLONIMSKI, PP ;
DUJARDIN, G .
MOLECULAR & GENERAL GENETICS, 1993, 240 (03) :419-427