Rapid kinetics of second messenger production in bitter taste

被引:69
作者
Spielman, AI
Nagai, H
Sunavala, G
Dasso, M
Breer, H
Boekhoff, I
Huque, T
Whitney, G
Brand, JG
机构
[1] UNIV PENN, SCH DENT MED, DEPT BIOCHEM, PHILADELPHIA, PA 19104 USA
[2] UNIV PENN, MONELL CHEM SENSES CTR, PHILADELPHIA, PA 19104 USA
[3] VET AFFAIRS MED CTR, PHILADELPHIA, PA 19104 USA
[4] FLORIDA STATE UNIV, DEPT PSYCHOL, TALLAHASSEE, FL 32306 USA
[5] SUNTORY LTD, INST FUNDAMENTAL RES, OSAKA 618, JAPAN
[6] UNIV STUTTGART HOHENHEIM, DEPT ZOOPHYSIOL, W-7000 STUTTGART 70, GERMANY
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 03期
关键词
gustatory; quench flow; G proteins;
D O I
10.1152/ajpcell.1996.270.3.C926
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The tasting of bitter compounds may have evolved as a protective mechanism against ingestion of potentially harmful substances. We have identified second messengers involved in bitter taste and show here for the first time that they are rapid and transient. Using a quench-flow system, we have studied bitter taste signal transduction in a pair of mouse strains that differ in their ability to taste the bitter stimulus sucrose octaacetate (SOA); however, both strains taste the bitter agent denatonium. In both strains of mice, denatonium (10 mM) induced a transient and rapid increase in levels of the second messenger inositol 1,4,5-trisphosphate (IP3) with a maximal production near 75-100 ms after stimulation. In contrast, SOA (100 mu M) brought about a similar increase in IP3 only in SOA-taster mice. The response to SOA was potentiated in the presence of GTP (1 mu M) The GTP-enhanced SOA-response supports a G protein-mediated response for this bitter compound. The rapid kinetics, transient nature, and specificity of the bitter taste stimulus-induced IP3 formation are consistent with the role of IP3 as a second messenger in the chemoelectrical transduction of bitter taste.
引用
收藏
页码:C926 / C931
页数:6
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