Direct electron transfer reaction of ascorbate oxidase immobilized by a self-assembled monolayer and polymer membrane combined system

被引：16

作者：

Murata, Kenichi ^{[1
]}

Nakamura, Nobuhumi ^{[1
]}

Ohno, Hiroyuki ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

ELECTROANALYSIS | 2007年 / 19卷 / 05期

关键词：

ascorbate oxidase; direct electron transfer; poly(ethylene oxide); chemical modification; immobilization;

D O I：

10.1002/elan.200603758

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper reports a novel enzyme-immobilization method for the direct electron transfer (DET) reaction of ascorbate oxidase from Acremonium sp. HI-25 (ASOM). ASOM was adsorbed onto a gold electrode modified with a self-assembled monolayer (SAM) of alkanethiol derivatives and immobilized by a cationic polymer membrane and anionic omega-carboxyalkanethiol combined system. The redox responses of the immobilized ASOM were investigated by cyclic voltammetry. We found that the DET reaction of ASOM was facilitated by this novel immobilization. On the other hand, the redox responses of poly(ethylene oxide) (PEO)-modified ASOMs were also investigated. ASOM was modified with two types of PEO which possess straight chain-shaped (PEO2000) or comb-shaped conformation (PM67)As a result, the DET reactions of PEO-modified ASOMs were also facilitated by this immobilization method. We concluded that this immobilization method is effective for promoting the DET reaction of ASOMs.

引用

页码：530 / 534

页数：5

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