Computer-assisted analysis of radial symmetry in human airway epithelial cilia: Assessment of congenital ciliary defects in primary ciliary dyskinesia

被引:11
作者
Carson, JL
Hu, SCS
Collier, AM
机构
[1] Univ N Carolina, Ctr Environm Med & Lung Biol, Dept Pediat, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Ctr Environm Med & Lung Biol, Dept Cell Biol & Anat, Chapel Hill, NC 27599 USA
[3] Univ N Carolina, Dept Pediat, Chapel Hill, NC USA
[4] Univ N Carolina, Frank Porter Graham Child Dev Ctr, Ctr Environm Med & Lung Biol, Dept Pediat, Chapel Hill, NC USA
关键词
cilia; image processing; primary ciliary dyskinesia; respiratory disease;
D O I
10.1080/01913120050132903
中图分类号
TH742 [显微镜];
学科分类号
摘要
The ultrastructural analysis of cilia and diagnosis of primary ciliary dyskinesia (PCD) in biopsies of airway epithelium is sometimes confounded by poor sampling, inconsistencies in tissue quality and processing, and other technical problems. Although clinical findings may lead to a presumptive diagnosis, ultrastructural analysis of ciliary axonemes is the standard for confirmation of PCD. The ultrastructural features of the cilium when viewed in cross section by transmission electron microscopy confer a radial symmetry to the axoneme. Current digital image processing techniques can be applied to such images to reinforce signal, diminish noise, and confirm consistency of position of axonemal structures, a process that can augment ultrastructural analysis for PCD. In this study, computer-assisted digital image processing was used to evaluate cross sections of cilia in airway epithelial biopsies from patients previously diagnosed with PCD as well as in control subjects with normal cilia. These studies supported the original diagnoses and provided some new insights into axonemal organization in PCD. This technique is simple and may be useful in providing a supporting means for confirming or ruling out a diagnosis of PCD in cases that appear equivocal.
引用
收藏
页码:169 / 174
页数:6
相关论文
共 14 条
[1]  
AFZELIUS B A, 1976, Science (Washington D C), V193, P317
[2]   LACK OF DYNEIN ARMS IN IMMOTILE HUMAN SPERMATOZOA [J].
AFZELIUS, BA ;
ELIASSON, R ;
JOHNSEN, O ;
LINDHOLMER, C .
JOURNAL OF CELL BIOLOGY, 1975, 66 (02) :225-232
[3]  
AFZELIUS BA, 1990, J CELL SCI, V95, P207
[4]   CILIARY DYSMORPHOLOGY AND DYSFUNCTION - PRIMARY OR ACQUIRED [J].
BOAT, TF ;
CARSON, JL .
NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (24) :1700-1702
[5]   2 TYPES OF CHLAMYDOMONAS FLAGELLAR MUTANTS MISSING DIFFERENT COMPONENTS OF INNER-ARM DYNEIN [J].
KAMIYA, R ;
KURIMOTO, E ;
MUTO, E .
JOURNAL OF CELL BIOLOGY, 1991, 112 (03) :441-447
[6]  
Kartagener M., 1933, BEITR KLINTUBERK, V83, P489, DOI [DOI 10.1007/BF02141468, 10.1007/bf02141468]
[7]   METHODS FOR ENHANCEMENT OF IMAGE DETAIL AND ACCENTUATION OF STRUCTURE IN ELECTRON MICROSCOPY [J].
MARKHAM, R ;
FREY, S ;
HILLS, GJ .
VIROLOGY, 1963, 20 (01) :88-&
[8]   A NEW PUTATIVE CELLULOSE-SYNTHESIZING COMPLEX OF COLEOCHAETE-SCUTATA [J].
OKUDA, K ;
BROWN, RM .
PROTOPLASMA, 1992, 168 (1-2) :51-63
[9]   ABSENCE OF AXONEMAL ARMS IN NASAL MUCOSA CILIA IN KARTAGENERS SYNDROME [J].
PEDERSEN, H ;
MYGIND, N .
NATURE, 1976, 262 (5568) :494-495
[10]   RANDOM CILIARY ORIENTATION - A CAUSE OF RESPIRATORY-TRACT DISEASE [J].
RUTLAND, J ;
DEIONGH, RU .
NEW ENGLAND JOURNAL OF MEDICINE, 1990, 323 (24) :1681-1684