The utility of [11C] dihydrotetrabenazine positron emission tomography scanning in assessing β-cell performance after sleeve gastrectomy and duodenal-jejunal bypass

Background. The aim of this study was to evaluate the effect of sleeve gastrectomy (SG) and duodenal-jejunal bypass (1 113) on glucose homeostasis and to evaluate the utility of positron emission tomography (PET) scanning for assessing beta-cell mass. Methods. Goto-Kakizaki rats were, divided into 4 groups: control, sham, SG or DJB. Oral glucose tolerance, insulin, and glucagon.-like peptide-1 (GLP-1) were measured, before and after surgery. Before and 90 days after treatment, [C-11] DTBZ micro PET scanning was performed. Results. The control and sham animals gained more weight compared with SG and DJB animals (P <= .05). Compared with. control animals, the glucose area under the curve was lower in DJB animals 30 and 45 days after operations (P <= .05). At killing, GLP-1 levels were greater in the DJB group compared with sham and SG (P <= .05), whereas insulin levels were greater in both 1 113 and SG compared with sham (P <= 05). With PET scanning, the 90-day posttreatment mean vesicular monoamine transporter type 2 binding index was greatest. in the DJB animals (2.45) compared with SG (L 17), both of which were greater than baseline control animals (0.81). Conclusion. In type 2 diabetic rodents, DJB leads to improved glucose homeostasis and an increase in VMAT2 density as measured by PET scanning. (Surgery 2010;147:303-9.)