Cationic electrophoresis and electrotransfer of membrane glycoproteins

被引：20

作者：

Buxbaum, E ^{[1
]}

机构：

[1] Kuwait Univ, Fac Med, Dept Biochem, Safat 13110, Kuwait

来源：

ANALYTICAL BIOCHEMISTRY | 2003年 / 314卷 / 01期

关键词：

cetyltrimethylammonium bromide; electrophoresis; blotting; glycoproteins; Mdr1;

D O I：

10.1016/S0003-2697(02)00639-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sodium dodecyl sulfate electrophoresis of glycoproteins results in broad, fuzzy bands. The use of the positively charged detergent cetyltrimethylammonium bromide (CTAB) avoids this problem. Protocols for CTAB electrophoresis, electroblotting, and protein detection are presented. These methods were found useful in studies on the multiple drug resistance ATPase Mdr1. (C) 2003 Elsevier Science (USA). All rights reserved.

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页码：70 / 76

页数：7

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