Interaction of human DNA topoisomerase I with G-quartet structures

被引：105

作者：

Arimondo, PB

Riou, JF

Mergny, JL

Tazi, J

Sun, JS

Garestier, T

Hélène, C

机构：

[1] Museum Natl Hist Nat, Biophys Lab, CNRS,UMR 8646, INSERM,U201, F-75231 Paris 05, France

[2] Aventis, Ctr Rech Vitry Alfortville, F-94403 Vitry, France

[3] Inst Genet Mol Montpellier, CNRS, UMR 5535, F-34033 Montpellier, France

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 24期

关键词：

D O I：

10.1093/nar/28.24.4832

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Because of their role in the control of the topological state of DNA, topoisomerases are ubiquitous and vital enzymes, which participate in nearly all events related to DNA metabolism including replication and transcription, We show here that human topoisomerase I (Topo I) plays an unexpected role of 'molecular matchmaker' for G-quartet formation, G-quadruplexes are multi-stranded structures held together by square planes of four guanines ('G-quartets') interacting by forming Hoogsteen hydrogen bonds. Topo I is able to promote the formation of four-stranded intermolecular DNA structures when added to single-stranded DNA containing a stretch of at least five guanines, We provide evidence that these complexes are parallel G-quartet structures, mediated by tetrads of hydrogen-bonded guanine, In addition, Topo I binds specifically to pre-formed parallel and anti-parallel G4-DNA.

引用

页码：4832 / 4838

页数：7

共 38 条

[21] Mechanism of action of eukaryotic DNA topoisomerase I and drugs targeted to the enzyme [J].