RAPD analysis and flavonoid composition of Aconitum as an aid for taxonomic discrimination

被引：24

作者：

Fico, G

Spada, A

Braca, A

Agradi, E

Morelli, I

Tomé, F

机构：

[1] Univ Milan, Dipartimento Biol, I-20133 Milan, Italy

[2] Univ Pisa, Dipartimento Chim Bioorgan & Biofarm, I-56126 Pisa, Italy

[3] Univ Milan, Dipartimento Sci Farmacol, I-20133 Milan, Italy

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2003年 / 31卷 / 03期

关键词：

Aconitum napellus subsp tauricum; Aconitum napellus subsp neomontanum; Aconitum paniculatum; Aconitum vulparia; Ranunculaceae; RAPD analysis; PCO; flavonoids;

D O I：

10.1016/S0305-1978(02)00153-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The RAPD technique and phytochemical analysis, based on the investigation of flavonoid composition, were used to study Aconitum vulparia, A. paniculatum, A. napellus subsp. tauricum (from two different localities), and A. napellus subsp. neomontanum. Twenty primers were screened for the genetic analysis and 14 were selected providing 51 polymorphic bands. The phenogram based on UPGMA clustering of Jaccard coefficient revealed a clear division between yellowish and blue Aconitum plants and inside this second group A. paniculatum is clearly separated from all populations belonging to A. napellus group. Also the flavonoid profile well characterise all species and subspecies under study. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：293 / 301

页数：9

共 18 条

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Flavonoids, DNA and relationships of Itea and Pterostemon [J].