Identification of ubiquitin as an immunophilin

被引：9

作者：

Davis, DL ^{[1
]}

Soldin, SJ

机构：

[1] Salisbury State Univ, Dept Hlth Sci, Salisbury, MD 21801 USA

[2] Catholic Univ Amer, Dept Biol, Washington, DC 20064 USA

[3] Childrens Natl Med Ctr, Dept Lab Med, Washington, DC 20010 USA

[4] George Washington Univ, Sch Med, Dept Pediat, Washington, DC 20052 USA

[5] George Washington Univ, Sch Med, Dept Pathol, Washington, DC USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 277卷 / 02期

关键词：

ubiquitin; immunophilin; tacrolimus; sirolimus; cyclosporin A;

D O I：

10.1006/bbrc.2000.3684

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified an 8.4 kDa minor immunophilin from calf thymus and Jurkat T cells as ubiquitin. It binds tacrolimus and sirolimus with K-d's of 0.8 and 0.08 nM, respectively, The binding of this protein to cyclosporin A is negligible. Binding of tacrolimus to two commercial sources of ubiquitin, a bovine product, and a recombinant human ubiquitin, was also demonstrated after HPLC purification of the purchased preparations. (C) 2000 Academic Press.

引用

页码：325 / 329

页数：5

共 17 条

[1] ACTIVATION-INDUCED UBIQUITINATION OF THE T-CELL ANTIGEN RECEPTOR [J].

CENCIARELLI, C ;

HOU, D ;

HSU, KC ;

RELLAHAN, BL ;

WIEST, DL ;

SMITH, HT ;

FRIED, VA ;

WEISSMAN, AM .

SCIENCE, 1992, 257 (5071) :795-797

[2] THE UBIQUITIN-MEDIATED PROTEOLYTIC PATHWAY AND MECHANISMS OF ENERGY-DEPENDENT INTRACELLULAR PROTEIN-DEGRADATION [J].

CIECHANOVER, A ;

FINLEY, D ;

VARSHAVSKY, A .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1984, 24 (01) :27-53

[3] Structure and functions of the 20S and 26S proteasomes [J].

Coux, O ;

Tanaka, K ;

Goldberg, AL .

ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :801-847

[4] Biochemical characterization of the minor immunophilins [J].

Davis, DL ;

Murthy, JN ;

Soldin, SJ .

CLINICAL BIOCHEMISTRY, 2000, 33 (02) :81-87

[5] Minor immunophilin binding of tacrolimus and sirolimus metabolites [J].

Davis, DL ;

Murthy, JN ;

Gallant-Haidner, H ;

Yatscoff, RW ;

Soldin, SJ .

CLINICAL BIOCHEMISTRY, 2000, 33 (01) :1-6

[6] THE YEAST PLASMA-MEMBRANE URACIL PERMEASE IS STABILIZED AGAINST STRESS-INDUCED DEGRADATION BY A POINT MUTATION IN A CYCLIN-LIKE DESTRUCTION BOX [J].

GALAN, JM ;

VOLLAND, C ;

URBANGRIMAL, D ;

HAGUENAUERTSAPIS, R .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 201 (02) :769-775

[7]

HAAS AL, 1985, PREP BIOCHEM, V15, P49

[8] CALCINEURIN IS A COMMON TARGET OF CYCLOPHILIN-CYCLOSPORINE-A AND FKBP-FK506 COMPLEXES [J].

LIU, J ;

FARMER, JD ;

LANE, WS ;

FRIEDMAN, J ;

WEISSMAN, I ;

SCHREIBER, SL .

CELL, 1991, 66 (04) :807-815

[9] Signal transduction through NF-κB [J].

May, MJ ;

Ghosh, S .

IMMUNOLOGY TODAY, 1998, 19 (02) :80-88

[10]

SCHLESINGER DH, 1975, BIOCHEMISTRY-US, V14, P2214, DOI 10.1021/bi00681a026

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