Structure and function of the genes involved in mannitol, arabitol and glucitol utilization from Pseudomonas fluorescens DSM50106

A DNA fragment from Pseudomonas fluorescens DSM50106 containing the genes for the uptake and utilization of mannitol, arabitol and glucitol was cloned in Escherichia coli and sequenced. Seven open reading frames (mtlEFGKDYZ) were identified on the 10031 bp fragment. The deduced amino acid sequences of the first four open reading frames (mtlEFGK) revealed significant similarity to the components of the maltose transport system in E. coli and Salmonella typhimurium. The gene mtlD encoding a polyol dehydrogenase was located downstream of mtlK. The deduced proteins of the last two genes on the fragment showed a high similarity to a fructokinase from Vibrio alginolyticus (MtlZ) and a xylulose kinase from Streptomyces rubiginosus (MtlY), respectively. Both genes were expressed in E. coli. MtlZ phosphorylated fructose, glucose and glucitol whereas MtlY was highly specific for xylulose. Upstream of mtlE, a putative promoter/operater region was identified by promoter probe studies which was active in P. fluorescens but not in E. coli. (C) 1997 Elsevier Science B.V.