Morphological alteration of golgi apparatus and subcellular compartmentalization of TGF-β1 in Golgi apparatus in gerbils following transient forebrain ischemia

被引：42

作者：

Hu, Zhiping

Zeng, Liuwang ^{[1
]}

Xie, Lesi

Lu, Wei

Zhang, Jie

Li, Ting

Wang, Xiang

机构：

[1] Cent S Univ, Xiangya Hosp 2, Dept Neurol, Changsha 410011, Hunan, Peoples R China

[2] Cent S Univ, Xiangya Sch Med, Dept Anat, Changsha 410013, Hunan, Peoples R China

来源：

NEUROCHEMICAL RESEARCH | 2007年 / 32卷 / 11期

关键词：

TGF-beta; 1; Golgi apparatus (GA); gerbils;

D O I：

10.1007/s11064-007-9382-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Golgi apparatus (GA) is a very important organelle involved in the metabolism of numerous proteins. TGF-beta 1 plays an important role in supporting neuronal survival after ischemic insults. Little is known, however, about the morphological alteration of GA and subcellular compartmentalization of TGF-beta 1 in brain after ischemia. Therefore, our present study was designed to check for GA morphological alterations and TGF-beta 1 subcellular localization. GA immunoreactivities were examined in the somatosensory cortex of gerbils after 10 min transient forebrain ischemia. Confocal Immunofluorographs of TGF-beta 1 and TGN38 were also taken. Results indicated that no fragmentation of GA was found in gerbils of norm, shams and 6, 24 and 72 h postocclusion, but some of the cortical cells showed fragmentation of GA in gerbils 7 days postocclusion. TGF-beta 1 was colocalized with TGN38, a marker molecule for the GA. We conclude that there was morphological alterations of GA and TGF-beta 1 was present in GA in the somatosensory cortex after 10 min ischemia.

引用

页码：1927 / 1931

页数：5

共 25 条

[11] IDENTIFICATION, SEQUENCING AND EXPRESSION OF AN INTEGRAL MEMBRANE-PROTEIN OF THE TRANS-GOLGI NETWORK (TGN38) [J].