Distinctive patterns of histone H4 acetylation are associated with defined sequence elements within both heterochromatic and euchromatic regions of the human genome

被引：71

作者：

Johnson, CA

O'Neill, LP

Mitchell, A

Turner, BM ^{[1
]}

机构：

[1] Univ Birmingham, Sch Med, Dept Anat, Chromatin & Gene Express Grp, Birmingham B15 2TT, W Midlands, England

[2] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1093/nar/26.4.994

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pattern of histone H4 acetylation in different genomic regions has been investigated by immunoprecipitating oligonucleosomes from a human lymphoblastoid cell line with antibodies to H4 acetylated at lysines 5, 8, 12 or 16. DNA from antibody-bound or unbound chromatin was assayed by slot blotting. Pol I and pol II transcribed genes located in euchromatin were shown to have levers of H4 acetylation at lysines 5, 8 and 12 equivalent to those in input chromatin, but to be slightly enriched in H4 acetylated at lysine 16. In no case did the acetylation level correlate with actual or potential transcriptional activity. All acetylated histone H4 isoforms were depleted in non-coding, simple;repeat DNA in heterochromatin, though the extent of depletion varied with the type of heterochromatin and with the isoform, Two single copy genes that map within or adjacent to blocks of paracentric heterochromatin are depleted in H4 acetylated at lysines 5, 8 and 12, but not 16, Consensus sequences of repetitive elements of the Alu family (SINES, enriched in R bands) were associated with H4 that was more highly acetylated at all four lysines than input chromatin, while H4 associated with KpnI elements (LINES, enriched in G bands) was significantly underacetylated.

引用

页码：994 / 1001

页数：8

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[11] PURIFICATION OF CPG ISLANDS USING A METHYLATED DNA-BINDING COLUMN [J].