Determination of dioxin and dioxin-like compounds in sediments from UK estuaries using a bio-analytical approach: chemical-activated luciferase expression (CALUX) assay

被引：42

作者：

Hurst, MR ^{[1
]}

Balaam, J ^{[1
]}

Chan-Man, YL ^{[1
]}

Thain, JE ^{[1
]}

Thomas, KV ^{[1
]}

机构：

[1] Burnham Lab, CEFAS, Burnham on Crouch CM0 8HA, Essex, England

来源：

MARINE POLLUTION BULLETIN | 2004年 / 49卷 / 7-8期

关键词：

dioxins sediments; estuaries; CALUX; monitoring; United Kingdom;

D O I：

10.1016/j.marpolbul.2004.04.012

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The DR-CALUX assay has been utilised for the bio-analytical screening of a number of estuarine sediments for dioxin-like activity. Total sediment extracts (samples containing all extracted compounds) and cleaned-up extracts (samples with the most stable compounds isolated from the total extracts) were screened. The concentration of the stable dioxin-like compounds in the cleaned-up sediment extracts was between 1.0 and 106 pg TEQ(CALUX) g(-1) dry weight. The majority of sediments contained levels of dioxin-like compounds that were above concentrations that are considered to be a low risk to aquatic organisms. The CALUX bio-analytical approach showed some disparity with the traditional analytical approach. The reasons for these differences have been identified tentatively: firstly, the DR-CALUX assay responds to all dioxin-like compounds, and secondly, it measures non-additive effects. The dioxin-like activity of compounds in sediment total extracts, which contain both labile and stable compounds, were also assessed and were six orders of magnitude higher than the cleaned-up samples. This suggests the vast majority of the total dioxin-like activity is attributable to labile compounds. Overall, the DR-CALUX assay is shown to be a useful tool in the assessment of dioxin-like activity in estuarine sediments. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：648 / 658

页数：11

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