Multi photon fluorescence microscopy: behavior of biological specimens under high intensity illumination

被引：4

作者：

Cheng, PC ^{[1
]}

Lin, BL ^{[1
]}

Kao, FJ ^{[1
]}

Sun, CK ^{[1
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Taipei 115, Taiwan

来源：

OPTICAL SENSING, IMAGING, AND MANIPULATION FOR BIOLOGICAL AND BIOMEDICAL APPLICATIONS | 2000年 / 4082卷

关键词：

multi-photon fluorescence microscopy; photon damage; cell damage; high intensity illumination; maize;

D O I：

10.1117/12.390536

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Recent development in multi-photon fluorescence microscopy, second and third harmonic generation microscopy (SHG and THG) and CARS open new dimensions in biological studies. Not only the technologies allow probing biological specimen both functionally and structurally with increasing spatial and temporal resolution, but also raise the interest in how biological specimens respond to high intensity illumination commonly used in these types of microscopy. We have used maize leaf protoplast as a model system to evaluate the photo-induced response of living sample under high intensity illumination. It was found that cells can be seriously damaged by high intensity NIR irradiation even the linear absorption coefficient is low in these wavelengths. Micro-spectroscopy of single chloroplast also allows us to gain insight on the possible photo-damage mechanism. In addition to fluorescence emission, second harmonic generation was observed in the maize protoplasts.

引用

页码：134 / 138

页数：5

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