Fosfomycin and rifampin disk diffusion tests for detection of Escherichia coli mutator strains

被引:15
作者
Galán, JC [1 ]
Tato, M [1 ]
Baquero, MR [1 ]
Turrientes, C [1 ]
Baquero, F [1 ]
Martinez, JL [1 ]
机构
[1] Ctr Nacl Biotecnol, Madrid, Spain
关键词
D O I
10.1128/JCM.42.9.4310-4312.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency greater than or equal to5 X 10(-7) was established at greater than or equal to70 and greater than or equal to20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 muI of an overnight culture. Strains with <30 and < 10 colonies in fosfomycin and rifampin inhibition zones are presumptively nonmutators.
引用
收藏
页码:4310 / 4312
页数:3
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