Bacterial components regulate the expression of toll-like receptor 4 on human mast cells

被引：37

作者：

Kubo, Y. ^{[1
]}

Fukuishi, N. ^{[1
]}

Yoshioka, M. ^{[1
]}

Kawasoe, Y. ^{[1
]}

Iriguchi, S. ^{[1
]}

Imajo, N. ^{[1
]}

Yasui, Y. ^{[1
]}

Matsui, N. ^{[1
]}

Akagi, M. ^{[1
]}

机构：

[1] Tokushima Bunri Univ, Fac Pharmaceut Sci, Dept Pharmacol, Tokushima 7708514, Japan

来源：

INFLAMMATION RESEARCH | 2007年 / 56卷 / 02期

关键词：

mast cells; TLRs; infection;

D O I：

10.1007/s00011-006-6064-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objectives and design: The aim of this study was to investigate whether the exposure of mast cells (MCs) to bacterial components affects the expression of Toll-like receptor (TLR) 4, and to elucidate the behavior of MCs during the early response to infection. Materials: Two human MC lines, HMC-1 and LAD2, were employed. Messenger RNA expression was observed by RT and real-time PCR. TLR4 expression was determined by Western blotting. TNF-alpha secretion was analyzed with ELISA. The degranulation ratio was measured with hetahexosaminidase assay. Results: Although bacterial components increased TLR4 mRNA, only lipopolysaccharide (LPS) augmented the TLR4 protein expression. LAD2 pre-treated with LPS for 8h resulted in 2-fold increased TNF-a secretion on LPS restimulation. Conclusion: These results suggest that the exposure of MCs to LPS may reinforce the innate immune system due to up-regulation of MC TLR4, followed by increased TNF-alpha release.

引用

页码：70 / 75

页数：6

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