Localization of chromosome breakpoints induced by DNase I in Chinese hamster ovary (CHO) cells

被引:23
作者
Folle, GA [1 ]
Boccardo, E [1 ]
Obe, G [1 ]
机构
[1] UNIV ESSEN GESAMTHSCH, DEPT GENET, D-45117 ESSEN, GERMANY
关键词
D O I
10.1007/s004120050260
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DNase I was electroporated into S-phase CHO cells and induced chromosome breakpoints were localized in G-banded metaphases. More than 75% of breakpoints mapped to Giemsa light bands, 18% to Giemsa-dark bands and about 7% to band junctions. Chromosome breakpoint clusters produced by DNase I colocalized with chromosome breakpoints induced by the restriction endonucleases AluI and BnmHI in the G1- and S-phases of the cell cycle in CHO cells, Digestion of metaphase spreads with AluI, BamHI and DNase I produced G-bands, indicating that G-light bands are more sensitive to endonuclease action. The possible role of nuclease-sensitive sites in active chromatin as selective targets for the induction of chromosome breakpoints by these endonucleases is discussed.
引用
收藏
页码:391 / 399
页数:9
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