Differential effects of culture conditions on the migration pattern of stromal cell-derived factor-stimulated hematopoietic stem cells

被引：18

作者：

Weidt, C

Niggemann, B

Hatzmann, W

Zänker, KS

Dittmar, T

机构：

[1] Univ Witten Herdecke, Inst Immunol, D-58448 Witten, Germany

[2] Univ Witten Herdecke, Marienhosp, Dept Gynecol, D-58448 Witten, Germany

来源：

STEM CELLS | 2004年 / 22卷 / 06期

关键词：

D O I：

10.1634/stemcells.22-6-890

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Evidence is mounting that hematopoietic stem cells (HSCs) play a critical role in bone marrow regeneration and tissue renewal, for which migration is an obvious prerequisite. Computer-aided analysis and a three-dimensional collagen matrix assay enabled us to analyze single-cell migratory characteristics of stromal cell-derived factor-la (SDF-1alpha)-stimulated cord blood-derived HSCs. We defined and resolved specific migratory parameters in spontaneous and SDF-1alpha-induced migration of these cells. The addition of interleukin 6 to the culture medium led to differential SDF-1alpha-stimulated migratory response, which comprised a recruitment of nonmoving cells and an increase in speed and frequency of pauses but a decrease in pause duration. We were thus able to decipher the exact parameters that result in an increase in the migration of HSCs and demonstrate that extensive analysis of single-cell behavior is elementary in the study of stem cell migration.

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收藏

页码：890 / 896

页数：7

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