Claudins in the tight junctions of stria vascularis marginal cells

被引:29
作者
Florian, P
Amasheh, S
Lessidrensky, M
Todt, I
Bloedow, A
Ernst, A
Fromm, M
Gitter, AH [1 ]
机构
[1] Free Univ Berlin, Klinikum Benjamin Franklin, Dept Clin Physiol, D-12200 Berlin, Germany
[2] UKB, Dept Otolaryngol, D-12683 Berlin, Germany
[3] Jena Univ Appl Sci, Dept Med Engn, D-07745 Jena, Germany
关键词
cochlea; inner ear; guinea pig; occludin; zonula occludens; epithelium; barrier function;
D O I
10.1016/S0006-291X(03)00498-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the mammalian cochlea, tight junctional strands are visible on freeze fracture images of marginal cells and other inner ear epithelia. The molecular composition of the strial tight junctions is, however, largely unknown. We investigated the expression of integral tight junction-proteins, claudin-1 to -4, and occludin, in stria vascularis of the guinea-pig cochlea, as compared to kidney. Western blot analysis revealed a strong expression of claudin-4 and occludin in strial tissue, and confocal immunofluorescence microscopy demonstrated their presence in the tight junctions of the marginal cells. In addition, a moderate level of claudin-3 and claudin-1 was detected and both were located in the marginal tight junctions. Claudins-1, -3, and -4 are characteristic of epithelia with low paracellular permeability and claudin-4 is known to restrict the passage of cations through epithelial tight junctions. In the marginal cells, these claudins appear to be responsible for the separation of the potassium-rich endolymph from the sodium-rich intrastrial fluid. In contrast, Western blot analysis and confocal microscopy demonstrated that the marginal cell epithelium does not contain claudin-2, which forms a cation-selective pore in tight junctions. Its absence indicates a cation-tight paracellular pathway in the marginal cells. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:5 / 10
页数:6
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