A mini exon in the sucrose:sucrose 1-fructosyltransferase gene of wheat

被引：11

作者：

Yoshida, M ^{[1
]}

Lin, D ^{[1
]}

Kawakami, A ^{[1
]}

机构：

[1] Natl Agr Res Ctr Hokkaido Reg, Sapporo, Hokkaido 0628555, Japan

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2004年 / 161卷 / 11期

关键词：

fructan; invertase; mini exon; sucrose : sucrose 1-fructosyltransferase;

D O I：

10.1016/j.jplph.2004.05.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We previously reported the cloning of a wheat sucrose: sucrose 1-fructosyltransferase (1-SST) cDNA, designated wft2. Wft2 proteins have fructosyltransferase enzyme activity and initiate fructan synthesis (Biosci. Biotechnol. Biochem. 66 (2002) 2297). In the current study, we cloned a genomic DNA fragment carrying the full-length 1-SST gene from winter wheat (Triticum aestivum). The genomic 1-SST gene is 3326 bp in length and contains four exons and three introns. Exon 2 has only 9 bp. This sequence encodes a part of a beta-fructosidase motif (NDPNG), a highly conserved motif found in plant invertases. This is the first report of a mini exon, one of the smallest exons known in plants, being found in a genomic 1-SST gene. (C) 2004 Elsevier GmbH. All rights reserved.

引用

页码：1277 / 1279

页数：3

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