Isolation and characterisation of a sucrose:sucrose 1-fructosyltransferase gene from perennial ryegrass (Lolium perenne)

被引：46

作者：

Chalmers, J

Johnson, X

Lidgett, A

Spangenberg, G ^{[1
]}

机构：

[1] Agr Victoria, Ctr Plant Biotechnol, Dept Primary Ind, Bundoora, Vic 3086, Australia

[2] La Trobe Univ, CRC Mol Plant Breeding, Bundoora, Vic 3086, Australia

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2003年 / 160卷 / 11期

关键词：

fructan biosynthesis; fructosyltransferase; Lolium perenne; perennial ryegrass; sucrose : sucrose 1-fructosyltransferase;

D O I：

10.1078/0176-1617-01107

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A sucrose: sucrose 1-fructosyltransferase (1-SST) gene and c;DNA (Lp1-SST) from perennial ryegrass (Lolium perenne) were isolated. The Lp1-SST gene was fully sequenced and shown to contain three exons and two introns. Nucleotide sequence analysis of the 4824 bp Lp1-SST genomic; sequence revealed 1618 bp of 5' UTR and an open reading frame of 1962 bp encoding a protein of 653 amino acids. Lp1-SST is 95% identical to the tall fescue 1-SST and contains plant fructosyltransferase functional domains. Lp1-SST corresponds to a single copy gene in perennial ryegrass, and is expressed in young leaf bases and mature leaf sheaths. The recombinant Lp1-SST protein from corresponding cDNA expression in Pichia pastoris showed 1-SST activity.

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收藏

页码：1385 / 1391

页数：7

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