Options and considerations when selecting a quantitative proteomics strategy

被引：448

作者：

Domon, Bruno ^{[1
]}

Aebersold, Ruedi ^{[1
,2
]}

机构：

[1] ETH, Inst Mol Syst Biol, Zurich, Switzerland

[2] Univ Zurich, Fac Sci, Zurich, Switzerland

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 07期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

PROTEIN-SEQUENCE ANALYSIS; MASS-SPECTROMETRY; YEAST PROTEOME; PEPTIDE; PLASMA; QUANTIFICATION; PERFORMANCE; MS;

D O I：

10.1038/nbt.1661

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The vast majority of proteomic studies to date have relied on mass spectrometric techniques to identify, and in some cases quantify, peptides that have been generated by proteolysis. Current approaches differ in the types of instrument used, their performance profiles, the manner in which they interface with biological research strategies, and their reliance on and use of prior information. Here, we consider the three main mass spectrometry (MS)-based proteomic approaches used today: shotgun (or discovery), directed and targeted strategies. We discuss the principles of each technique, their strengths and weaknesses and the dependence of their performance profiles on the composition of the biological sample. Our goal is to provide a rational framework for selecting strategies optimally suited to address the specific research issue under consideration.

引用

页码：710 / 721

页数：12

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