Identification of proliferation-induced genes in Arabidopsis thaliana -: Characterization of a new member of the highly evolutionarily conserved histone H2A.F/Z variant subfamily

被引：23

作者：

Callard, D

Mazzolini, L ^{[1
]}

机构：

[1] INRA, Lab Biol Mol Relat Plantes Microorganismes, CNRS, Unite Mixte Rech 215, F-31326 Castanet Tolosan, France

[2] Inst Pharmacol & Biol Struct, CNRS, Unite Propre Rech 9062, F-31077 Toulouse, France

来源：

PLANT PHYSIOLOGY | 1997年 / 115卷 / 04期

关键词：

D O I：

10.1104/pp.115.4.1385

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The changes in gene expression associated with the reinitiation of cell division and subsequent progression through the cell cycle in Arabidopsis thaliana cell-suspension cultures were investigated. Partial synchronization of cells was achieved by a technique combining phosphate starvation and a transient treatment with the DNA replication inhibitor aphidicolin. Six cDNAs corresponding to genes highly induced in proliferating cells and showing cell-cycle-regulated expression were obtained by the mRNA differential display technique. Full-length cDNA clones (cH2BAt and cH2AvAt) corresponding to two of the display products were subsequently isolated. The cH2BAt clone codes for a novel histone H2B protein, whereas the cH2AvAt cDNA corresponds to a gene encoding a new member of the highly conserved histone H2A.F/Z subfamily of chromosomal proteins. Further studies indicated that H2AvAt mRNA expression is tightly correlated with cell proliferation in cell-suspension cultures, and that closely related analogs of the encoded protein exist in Arabidopsis. The implications of the conservation of histone H2A.F/Z variants in plants are discussed.

引用

页码：1385 / 1395

页数：11

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