Hydrogen peroxide is not released following reaction of cyanide with several catalytically important derivatives of cytochrome c oxidase

被引：15

作者：

Fabian, M

Palmer, G

机构：

[1] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

[2] Slovak Acad Sci, Inst Expt Phys, Kosice 04353, Slovakia

来源：

FEBS LETTERS | 1998年 / 422卷 / 01期

关键词：

cytochrome c oxidase; peroxy derivative; oxyferryl derivative; hydrogen peroxide; cyanide;

D O I：

10.1016/S0014-5793(97)01561-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have looked for the production of hydrogen peroxide following reaction of oxidized cytochrome c oxidase and two oxy derivatives (compounds P and F) with cyanide. In each case the final product was the cyanide adduct of cytochrome c oxidase. In no case release of hydrogen peroxide was detected, as gauged by the scopoletin plus horse radish peroxidase assay. The simplest conclusion is that none of these forms of the enzyme contains intact hydrogen peroxide. (C) 1998 Federation of European Biochemical Societies.

引用

页码：1 / 4

页数：4