Dynamic addressing of a surface pattern by a stimuli-responsive fusion protein

被引：38

作者：

Frey, W ^{[1
]}

Meyer, DE ^{[1
]}

Chilkoti, A ^{[1
]}

机构：

[1] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA

来源：

ADVANCED MATERIALS | 2003年 / 15卷 / 03期

关键词：

D O I：

10.1002/adma.200390058

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thermodynamically reversible addressing of proteins (TRAP) is demonstrated. A stimuli-responsive elastin-like polypeptide fused to thioredoxin is used to reversibly address the fusion protein onto a chemically micropatterned surface template by switching the interaction between the surface and the stimuli-responsive polypeptide (see Figure).

引用

收藏

页码：248 / +

页数：5

相关论文

共 26 条

[1] Protein patterning [J].

Blawas, AS ;

Reichert, WM .

BIOMATERIALS, 1998, 19 (7-9) :595-609

[2] Geometric control of cell life and death [J].

Chen, CS ;

Mrksich, M ;

Huang, S ;

Whitesides, GM ;

Ingber, DE .

SCIENCE, 1997, 276 (5317) :1425-1428

[3] VERSATILE ESCHERICHIA-COLI THIOREDOXIN SPECIFIC MONOCLONAL-ANTIBODIES AFFORD CONVENIENT ANALYSIS AND PURIFICATION OF PROKARYOTE EXPRESSED SOLUBLE FUSION PROTEIN [J].

DICKASON, RR ;

EDWARDS, RA ;

BRYAN, J ;

HUSTON, DP .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 185 (02) :237-244

[4] PATTERNING SELF-ASSEMBLED MONOLAYERS - APPLICATIONS IN MATERIALS SCIENCE [J].

KUMAR, A ;

BIEBUYCK, HA ;

WHITESIDES, GM .

LANGMUIR, 1994, 10 (05) :1498-1511

[5] Self-assembled monolayers of alkanethiolates presenting mannitol groups are inert to protein adsorption and cell attachment [J].

Luk, YY ;

Kato, M ;

Mrksich, M .

LANGMUIR, 2000, 16 (24) :9604-9608

[6]

MacBeath G, 2000, SCIENCE, V289, P1760

[7] Protein purification by fusion with an environmentally responsive elastin-like polypeptide: Effect of polypeptide length on the purification of thioredoxin [J].

Meyer, DE ;

Trabbic-Carlson, K ;

Chilkoti, A .

BIOTECHNOLOGY PROGRESS, 2001, 17 (04) :720-728

[8] Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: Examples from the elastin-like polypeptide system [J].

Meyer, DE ;

Chilkoti, A .

BIOMACROMOLECULES, 2002, 3 (02) :357-367

[9] Purification of recombinant proteins by fusion with thermally-responsive polypeptides [J].

Meyer, DE ;

Chilkoti, A .

NATURE BIOTECHNOLOGY, 1999, 17 (11) :1112-1115

[10] A perspective on protein microarrays [J].

Mitchell, P .

NATURE BIOTECHNOLOGY, 2002, 20 (03) :225-229