Hemagglutinin-Pseudotyped Green Fluorescent Protein-Expressing Influenza Viruses for the Detection of Influenza Virus Neutralizing Antibodies

被引：78

作者：

Martinez-Sobrido, Luis ^{[1
]}

Cadagan, Richard ^{[1
]}

Steel, John ^{[1
]}

Basler, Christopher F. ^{[1
]}

Palese, Peter ^{[1
,2
]}

Moran, Thomas M. ^{[1
]}

Garcia-Sastre, Adolfo ^{[1
,2
,3
]}

机构：

[1] Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA

[2] Mt Sinai Sch Med, Dept Med, New York, NY 10029 USA

[3] Mt Sinai Sch Med, Global Hlth & Emerging Pathogens Inst, New York, NY 10029 USA

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 04期

关键词：

PANDEMIC INFLUENZA; A VIRUSES; H5N1; COMBINATION; MORTALITY; VECTOR;

D O I：

10.1128/JVI.01433-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Influenza virus is a highly contagious virus that causes yearly epidemics and occasional pandemics of great consequence. Influenza virus neutralizing antibodies (NAbs) are promising prophylactic and therapeutic reagents. Detection of NAbs in serum samples is critical to evaluate the prevalence and spread of new virus strains. Here we describe the development of a simple, sensitive, specific, and safe screening assay for the rapid detection of NAbs against highly pathogenic influenza viruses under biosafety level 2 (BSL-2) conditions. This assay is based on the use of influenza viruses in which the hemagglutinin (HA) gene is replaced by a gene expressing green fluorescent protein (GFP). These GFP-expressing influenza viruses replicate to high titers in HA-expressing cell lines, but in non-HA-expressing cells, their replication is restricted to a single cycle.

引用

页码：2157 / 2163

页数：7

共 21 条

[1] Characterization of a trypsin-dependent avian influenza H5N1-pseudotyped HIV vector system for high throughput screening of inhibitory molecules [J].

Ao, Zhujun ;

Patel, Ami ;

Tran, Kaylie ;

He, Xinying ;

Fowke, Keith ;

Coombs, Kevin ;

Kobasa, Darwyn ;

Kobinger, Gary ;

Yao, Xiaojian .

ANTIVIRAL RESEARCH, 2008, 79 (01) :12-18

[2] Brief report - Oseltamivir resistance during treatment of influenza A (H5N1) infection [J].

de Jong, MD ;

Thanh, TT ;

Khanh, TH ;

Hien, VM ;

Smith, GJD ;

Chau, NV ;

Cam, BV ;

Qui, PT ;

Ha, DQ ;

Guan, Y ;

Peiris, JSM ;

Hien, TT ;

Farrar, J .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (25) :2667-2672

[3] Antigenic and Genetic Characteristics of Swine-Origin 2009 A(H1N1) Influenza Viruses Circulating in Humans [J].

Garten, Rebecca J. ;

Davis, C. Todd ;

Russell, Colin A. ;

Shu, Bo ;

Lindstrom, Stephen ;

Balish, Amanda ;

Sessions, Wendy M. ;

Xu, Xiyan ;

Skepner, Eugene ;

Deyde, Varough ;

Okomo-Adhiambo, Margaret ;

Gubareva, Larisa ;

Barnes, John ;

Smith, Catherine B. ;

Emery, Shannon L. ;

Hillman, Michael J. ;

Rivailler, Pierre ;

Smagala, James ;

de Graaf, Miranda ;

Burke, David F. ;

Fouchier, Ron A. M. ;

Pappas, Claudia ;

Alpuche-Aranda, Celia M. ;

Lopez-Gatell, Hugo ;

Olivera, Hiram ;

Lopez, Irma ;

Myers, Christopher A. ;

Faix, Dennis ;

Blair, Patrick J. ;

Yu, Cindy ;

Keene, Kimberly M. ;

Dotson, P. David, Jr. ;

Boxrud, David ;

Sambol, Anthony R. ;

Abid, Syed H. ;

George, Kirsten St. ;

Bannerman, Tammy ;

Moore, Amanda L. ;

Stringer, David J. ;

Blevins, Patricia ;

Demmler-Harrison, Gail J. ;

Ginsberg, Michele ;

Kriner, Paula ;

Waterman, Steve ;

Smole, Sandra ;

Guevara, Hugo F. ;

Belongia, Edward A. ;

Clark, Patricia A. ;

Beatrice, Sara T. ;

Donis, Ruben .

SCIENCE, 2009, 325 (5937) :197-201

[4] Updating the accounts: global mortality of the 1918-1920 "Spanish" influenza pandemic [J].

Johnson, NPAS ;

Mueller, J .

BULLETIN OF THE HISTORY OF MEDICINE, 2002, 76 (01) :105-115

[5] Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies [J].

Kashyap, Arun K. ;

Steel, John ;

Oner, Ahmet F. ;

Dillon, Michael A. ;

Swale, Ryann E. ;

Wall, Katherine M. ;

Perry, Kimberly J. ;

Faynboym, Aleksandr ;

Illhan, Mahmut ;

Horowitz, Michael ;

Horowitz, Lawrence ;

Palese, Peter ;

Bhatt, Ramesh R. ;

Lerner, Richard A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (16) :5986-5991

[6] Resistant influenza A viruses in children treated with oseltarnivir: descriptive study [J].

Kiso, M ;

Mitamura, K ;

Sakai-Tagawa, Y ;

Shiraishi, K ;

Kawakami, C ;

Kimura, K ;

Hayden, FG ;

Sugaya, N ;

Kawaoka, Y .

LANCET, 2004, 364 (9436) :759-765

[7] Protective immunity to lethal challenge of the 1918 pandemic influenza virus by vaccination [J].

Kong, Wing-pui ;

Hood, Chantelle ;

Yang, Zhi-yong ;

Wei, Chih-Jen ;

Xu, Ling ;

Garcia-Sastre, Adolfo ;

Tumpey, Terrence M. ;

Nabel, Gary J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (43) :15987-15991

[8] Pandemic Influenza: An Inconvenient Mutation [J].

Layne, Scott P. ;

Monto, Arnold S. ;

Taubenberger, Jeffery K. .

SCIENCE, 2009, 323 (5921) :1560-1561

[9] Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia [J].

Li, KS ;

Guan, Y ;

Wang, J ;

Smith, GJD ;

Xu, KM ;

Duan, L ;

Rahardjo, AP ;

Puthavathana, P ;

Buranathai, C ;

Nguyen, TD ;

Estoepangestie, ATS ;

Chaisingh, A ;

Auewarakul, P ;

Long, HT ;

Hanh, NTH ;

Webby, RJ ;

Poon, LLM ;

Chen, H ;

Shortridge, KF ;

Yuen, KY ;

Webster, RG ;

Peiris, JSM .

NATURE, 2004, 430 (6996) :209-213

[10] Use of pseudotyped retroviral vectors to analyze the receptor-binding pocket of hemagglutinin from a pathogenic avian influenza A virus (H7 subtype) [J].

Lin, AH ;

Cannon, PM .

VIRUS RESEARCH, 2002, 83 (1-2) :43-56

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