Latex-functionalized monolithic columns for the separation of carbohydrates by micro anion-exchange chromatography

被引：104

作者：

Hilder, EF

Svec, F ^{[1
]}

Fréchet, JMJ

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Mat Sci, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1053卷 / 1-2期

关键词：

monolithic columns; stationary phases; LC; carbohydrates; latex;

D O I：

10.1016/j.chroma.2004.06.125

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel stationary phase for micro ion chromatography has been prepared by coating a porous poly(butyl methacrylate-co-ethylene dimethacrylate-co-2-acrylamido-2-methyl-1-propanesulfonic acid) monolith with quaternary amine-functionalized latex particles via simple electrostatic binding. This stationary phase enabled the separation of saccharides in the mobile phase with a high-pH value consisting of aqueous ammonia solution in anion-exchange mode using evaporative light scattering for detection. Effects of both porous properties of the monolithic stationary phase and chromatographic conditions on the separation ability were studied. Under optimized conditions, an efficient separation of seven saccharides was achieved in less than 10 min. The stationary phase also enables the separation of saccharides obtained by the enzymatic hydrolysis of corn starch. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：101 / 106

页数：6

共 23 条

[11] Molded rigid polymer monoliths as separation media for capillary electrochromatography.: 1.: Fine control of porous properties and surface chemistry [J].