Spontaneous curvature of phosphatidic acid and lysophosphatidic acid

被引:218
作者
Kooijman, EE
Chupin, V
Fuller, NL
Kozlov, MM
de Kruijff, B
Burger, KNJ
Rand, PR
机构
[1] Univ Utrecht, Dept Membrane Biochem, Ctr Biomembranes & Lipid Enzymol, NL-3584 CH Utrecht, Netherlands
[2] Brock Univ, St Catharines, ON L2S 3A1, Canada
[3] Tel Aviv Univ, Sackler Fac Med, Dept Physiol & Pharmacol, IL-69978 Tel Aviv, Israel
[4] Univ Utrecht, Dept Mol Cell Biol, Biomembrane Inst, NL-3584 CH Utrecht, Netherlands
关键词
D O I
10.1021/bi0478502
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The formation of phosphatidic acid (PA) from lysophosphatidic acid (LPA), diacylglycerol, or phosphatidylcholine plays a key role in the regulation of intracellular membrane fission events, but the underlying molecular mechanism has not been resolved. A likely possibility is that PA affects local membrane curvature facilitating membrane bending and fission. To examine this possibility, we determined the spontaneous radius of curvature (R-0p) of PA and LPA, carrying oleoyl fatty acids, using well-established X-ray diffraction methods. We found that, under physiological conditions of pH and salt concentration (pH 7.0, 150 mM NaCl), the R-0p values of PA and LPA were -46 Angstrom and +20 Angstrom, respectively. Thus PA has considerable negative spontaneous curvature while LPA has the most positive spontaneous curvature of any membrane lipid measured to date. The further addition of Ca2+ did not significantly affect lipid spontaneous curvature; however, omitting NaCl from the hydration buffer greatly reduced the spontaneous curvature of PA, turning it into a cylindrically shaped lipid molecule (R-0p of -1.3 x 10(2) Angstrom). Our quantitative data on the spontaneous radius of curvature of PA and LPA at a physiological pH and salt concentration will be instrumental in developing future models of biomembrane fission.
引用
收藏
页码:2097 / 2102
页数:6
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