Marfey's reagent: Past, present, and future uses of 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide

被引：84

作者：

B'Hymer, C ^{[1
]}

Montes-Bayon, M ^{[1
]}

Caruso, JA ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA

来源：

JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 1-2期

关键词：

pre-column derivatization; Marfey's reagent; FDAA; 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide; LIQUID-CHROMATOGRAPHIC SEPARATION; AMINO-ACID ENANTIOMERS; CAPILLARY GAS-CHROMATOGRAPHY; OPTICAL ISOMERS; CHIRAL SPECIATION; DERIVATIZATION; RESOLUTION; HPLC; CYCLODEXTRIN; PURITY;

D O I：

10.1002/jssc.200390019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes some of the uses of Marfey's reagent, 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA), a pre-column derivatizing reagent for the separation of enantiomeric isomers of amino acids and amine compounds. An introduction to the basic implementation of this reagent and its historical development are presented. Actual uses in amino acid, short peptide, and pharmaceutical compounds are included, as well as the advantages and disadvantages over other pre-column derivatization techniques and direct chromatographic separations. Applications of current interest, including its use as an orthogonal analysis and in enantiomeric purity analysis of selenoamino acids using element specific detection, are also discussed.

引用

页码：7 / 19

页数：13

共 102 条

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