Structural determination of novel lacto-N-decaose and its monofucosylated analogue from human milk by electrospray tandem mass spectrometry and 1H NMR spectroscopy

被引：38

作者：

Chai, WG

Piskarev, VE

Zhang, YB

Lawson, AM

Kogelberg, H

机构：

[1] Imperial Coll Fac Med, MRC Glycosci Lab, Harrow HA1 3UJ, Middx, England

[2] Russian Acad Sci, AN Nesmeyanov Organoelement Cpds Inst, Moscow 117813, Russia

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2005年 / 434卷 / 01期

基金：

英国医学研究理事会;

关键词：

electrospray mass spectrometry; tandem mass spectrometry; NMR human milk; oligosaccharide; lacto-N-decaose; Lewis antigen;

D O I：

10.1016/j.abb.2004.09.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and characterised two neutral oligosaccharides, one nonfucosylated and the other monofucosylated, from human milk that are based on the doubly branched lacto-N-decaose core. Their structures have been determined by a combined use of electrospray tandem mass spectrometry (ES-MS/MS) and NMR spectroscopy. The sequences of the three branches resulted from the double-branching, including the identity and location of the blood-group-related Lewis determinant and partial linkages, were elucidated by the unique method of high sensitivity negative-ion ES-MS/MS analysis. Their full Structure assignment was completed by methylation analysis and H-1 NMR. The monofucosylated lacto-N-decaose, Galbeta1-4(Fucalpha1-3)GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)-Galbeta1-4GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4Glc is a novel sequence, whereas the nonfucosylated lacto-N-decaose, Galbeta1-4GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4GlcNAcbeta1-6(Galbeta1-3GlcNAcbeta1-3)Galbeta1-4Glc, has not been isolated and identified as an individual oligosaccharide. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：116 / 127

页数：12

共 24 条

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