PRIMARY STRUCTURE OF HUMAN-MILK OCTASACCHARIDES, DODECASACCHARIDES AND TRIDECASACCHARIDES DETERMINED BY A COMBINATION OF H-1-NMR SPECTROSCOPY AND FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY - EVIDENCE FOR A NEW CORE STRUCTURE, THE PARA-LACTO-N-OCTAOSE

被引：44

作者：

HAEUWFIEVRE, S ^{[1
]}

WIERUSZESKI, JM ^{[1
]}

PLANCKE, Y ^{[1
]}

MICHALSKI, JC ^{[1
]}

MONTREUIL, J ^{[1
]}

STRECKER, G ^{[1
]}

机构：

[1] UNIV LILLE 1,CHIM BIOL LAB,CNRS,UNITE MIXTE 111,F-59655 VILLENEUVE DASCQ,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 215卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb18042.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two octasaccharides, two dodecasaccharides and a tridecasaccharide have been isolated from human milk by a combination of paper chromatography, high-performance liquid chromatography and high-performance anion-exchange chromatography. Their structural analysis investigated by 400-MHz H-1- and C-13-NMR spectroscopy and by mass spectrometry led to the following structures. [GRAPHICS] The two octasaccharides have been previously characterized in human milk; the complete assignment of the H-1 and C-13 spectra is reported here. One of the dodecasaccharides is a tetrafucosyl derivative with a new core: typeI-(beta1-3)-typeII-(beta1-3)-typeII-(beta1-3)-Gal((beta1-4)Glc, where typeI = Gal(beta1-3)GlcNAc and typeII = Gal(beta1-4)GIcNAc. These oligosaccharides display simultaneously Lewis(b) and Lewis(x) determinants or Lewis(a), Lewis(b) and Lewis(x) determinants.

引用

页码：361 / 371

页数：11

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